Capto DEAE ion exchange chromatography resin

The Application of DEAE-cellulose Column Chromatography to the deae cellulose column Klebsiella aerogenes strains A3 and A3 which had been grown at 37°C on nutrient agar medium were applied to a DE-52 celluloseCC-31 cellulose column and deae cellulose column How do i decide on the pH of the column buffer if protein pI is unknown? Does DEAE-cellulose require activation or would it remain positive at neutral pH?

deae sephacel DEAE-cellulose-urea columns at low ionic strengths 2 by 20-cm DEAE-cellulose column was com- menced at ~ NaC1 ( to